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11  structures 1831  species 0  interactions 14283  sequences 371  architectures

Family: Methyltransf_16 (PF10294)

Summary: Lysine methyltransferase

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

The Pfam group coordinates the annotation of Pfam families in Wikipedia, but we have not yet assigned a Wikipedia article to this family. If you think that a particular Wikipedia article provides good annotation, please let us know.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

Lysine methyltransferase Provide feedback

Methyltrans_16 is a lysine methyltransferase. Characterised members of this family are protein methyltransferases targetting Lys residues in specific proteins, including calmodulin, VCP, Kin17 and Hsp70 proteins [2,3,4,5].

Literature references

  1. Katz JE, Dlakic M, Clarke S; , Mol Cell Proteomics 2003;2:525-540.: Automated identification of putative methyltransferases from genomic open reading frames. PUBMED:12872006 EPMC:12872006

  2. Magnani R, Dirk LM, Trievel RC, Houtz RL;, Nat Commun. 2010;1:43.: Calmodulin methyltransferase is an evolutionarily conserved enzyme that trimethylates Lys-115 in calmodulin. PUBMED:20975703 EPMC:20975703

  3. Kernstock S, Davydova E, Jakobsson M, Moen A, Pettersen S, Maelandsmo GM, Egge-Jacobsen W, Falnes PO;, Nat Commun. 2012;3:1038.: Lysine methylation of VCP by a member of a novel human protein methyltransferase family. PUBMED:22948820 EPMC:22948820

  4. Cloutier P, Lavallee-Adam M, Faubert D, Blanchette M, Coulombe B;, PLoS Genet. 2013;9:e1003210.: A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity. PUBMED:23349634 EPMC:23349634

  5. Jakobsson ME, Moen A, Bousset L, Egge-Jacobsen W, Kernstock S, Melki R, Falnes PO;, J Biol Chem. 2013;288:27752-27763.: Identification and characterization of a novel human methyltransferase modulating Hsp70 protein function through lysine methylation. PUBMED:23921388 EPMC:23921388


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR019410

This entry represents a group of lysine methyltransferases. Characterised members of this family are protein methyltransferases targetting Lys residues in specific proteins, including calmodulin, VCP, Kin17 and Hsp70 proteins [ PUBMED:20975703 , PUBMED:22948820 , PUBMED:23349634 , PUBMED:23921388 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan NADP_Rossmann (CL0063), which has the following description:

A class of redox enzymes are two domain proteins. One domain, termed the catalytic domain, confers substrate specificity and the precise reaction of the enzyme. The other domain, which is common to this class of redox enzymes, is a Rossmann-fold domain. The Rossmann domain binds nicotinamide adenine dinucleotide (NAD+) and it is this cofactor that reversibly accepts a hydride ion, which is lost or gained by the substrate in the redox reaction. Rossmann domains have an alpha/beta fold, which has a central beta sheet, with approximately five alpha helices found surrounding the beta sheet.The strands forming the beta sheet are found in the following characteristic order 654123. The inter sheet crossover of the stands in the sheet form the NAD+ binding site [1]. In some more distantly relate Rossmann domains the NAD+ cofactor is replaced by the functionally similar cofactor FAD.

The clan contains the following 206 members:

2-Hacid_dh_C 3Beta_HSD 3HCDH_N 3HCDH_RFF adh_short adh_short_C2 ADH_zinc_N ADH_zinc_N_2 AdoHcyase_NAD AdoMet_MTase AlaDh_PNT_C Amino_oxidase ApbA AviRa B12-binding Bac_GDH Bin3 Bmt2 CbiJ CheR CMAS CmcI CoA_binding CoA_binding_2 CoA_binding_3 Cons_hypoth95 CoV_ExoN CoV_Methyltr_2 DAO DapB_N DFP DNA_methylase DOT1 DRE2_N DREV DUF1442 DUF1611_N DUF166 DUF1776 DUF2431 DUF268 DUF2855 DUF3410 DUF364 DUF43 DUF5129 DUF5130 DUF6094 DUF938 DXP_reductoisom DXPR_C Eco57I ELFV_dehydrog Eno-Rase_FAD_bd Eno-Rase_NADH_b Enoyl_reductase Epimerase F420_oxidored FAD_binding_2 FAD_binding_3 FAD_oxidored Fibrillarin FMO-like FmrO FtsJ fvmX7 G6PD_N GCD14 GDI GDP_Man_Dehyd GFO_IDH_MocA GIDA GidB GLF Glu_dehyd_C Glyco_hydro_4 Glyco_tran_WecG GMC_oxred_N Gp_dh_N GRAS GRDA HcgC HI0933_like HIM1 IlvN ISPD_C K_oxygenase KR LCM Ldh_1_N LpxI_N Lycopene_cycl Malic_M Mannitol_dh MCRA Met_10 Methyltr_RsmB-F Methyltr_RsmF_N Methyltrans_Mon Methyltrans_SAM Methyltransf_10 Methyltransf_11 Methyltransf_12 Methyltransf_14 Methyltransf_15 Methyltransf_16 Methyltransf_17 Methyltransf_18 Methyltransf_19 Methyltransf_2 Methyltransf_20 Methyltransf_21 Methyltransf_22 Methyltransf_23 Methyltransf_24 Methyltransf_25 Methyltransf_28 Methyltransf_29 Methyltransf_3 Methyltransf_30 Methyltransf_31 Methyltransf_32 Methyltransf_33 Methyltransf_34 Methyltransf_4 Methyltransf_5 Methyltransf_7 Methyltransf_8 Methyltransf_9 Methyltransf_PK MethyltransfD12 MetW Mg-por_mtran_C MOLO1 Mqo MT-A70 MTS Mur_ligase N2227 N6-adenineMlase N6_Mtase N6_N4_Mtase NAD_binding_10 NAD_binding_2 NAD_binding_3 NAD_binding_4 NAD_binding_5 NAD_binding_7 NAD_binding_8 NAD_binding_9 NAD_Gly3P_dh_N NAS NmrA NNMT_PNMT_TEMT NodS OCD_Mu_crystall Orbi_VP4 PALP PARP_regulatory PCMT PDH PglD_N Polysacc_syn_2C Polysacc_synt_2 Pox_MCEL Pox_mRNA-cap Prenylcys_lyase PrmA PRMT5 Pyr_redox Pyr_redox_2 Pyr_redox_3 Reovirus_L2 RmlD_sub_bind Rossmann-like rRNA_methylase RrnaAD Rsm22 RsmJ Sacchrp_dh_NADP SAM_MT SE Semialdhyde_dh Shikimate_DH Spermine_synth TehB THF_DHG_CYH_C Thi4 ThiF TPM_phosphatase TPMT TrkA_N TRM TRM13 TrmK tRNA_U5-meth_tr Trp_halogenase TylF Ubie_methyltran UDPG_MGDP_dh_N UPF0020 UPF0146 Urocanase V_cholerae_RfbT XdhC_C YjeF_N

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(13)
Full
(14283)
Representative proteomes UniProt
(31499)
RP15
(2956)
RP35
(7079)
RP55
(11562)
RP75
(15373)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(13)
Full
(14283)
Representative proteomes UniProt
(31499)
RP15
(2956)
RP35
(7079)
RP55
(11562)
RP75
(15373)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(13)
Full
(14283)
Representative proteomes UniProt
(31499)
RP15
(2956)
RP35
(7079)
RP55
(11562)
RP75
(15373)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_19672 (Release 21.0)
Previous IDs: none
Type: Family
Sequence Ontology: SO:0100021
Author: Wood V , Bateman A
Number in seed: 13
Number in full: 14283
Average length of the domain: 155.50 aa
Average identity of full alignment: 20 %
Average coverage of the sequence by the domain: 49.32 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 20.2 20.2
Trusted cut-off 20.2 20.2
Noise cut-off 20.1 20.1
Model length: 174
Family (HMM) version: 11
Download: download the raw HMM for this family

Species distribution

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Selections

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Methyltransf_16 domain has been found. There are 11 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0G2L4U4 View 3D Structure Click here
A0A0P0WZ67 View 3D Structure Click here
A0A0R0GVQ6 View 3D Structure Click here
A0A0R0H834 View 3D Structure Click here
A0A0R0IWS7 View 3D Structure Click here
A0A0R0K769 View 3D Structure Click here
A0A0R0KP13 View 3D Structure Click here
A0A0R4IMW2 View 3D Structure Click here
A0A1D6E7G4 View 3D Structure Click here
A0A1D6GLH0 View 3D Structure Click here
A0A1D6GYM8 View 3D Structure Click here
A0A1D6HPM6 View 3D Structure Click here
A0A1D6IGX7 View 3D Structure Click here
A0A1D6IKZ9 View 3D Structure Click here
A0A1D6J1W2 View 3D Structure Click here
A0A1D6JND8 View 3D Structure Click here
A0A1D6JNV1 View 3D Structure Click here
A0A1D6LAB0 View 3D Structure Click here
A0A1D6LAG0 View 3D Structure Click here
A0A1D6LQZ8 View 3D Structure Click here
A0A1D6QAT6 View 3D Structure Click here
A0A1D8PGX0 View 3D Structure Click here
A0A1P8AWF5 View 3D Structure Click here
A0A1P8BE87 View 3D Structure Click here
A0A2R8QKS9 View 3D Structure Click here
A2AA28 View 3D Structure Click here
A4HWH0 View 3D Structure Click here
A4HWH9 View 3D Structure Click here
A4HXF2 View 3D Structure Click here
A4I047 View 3D Structure Click here
A4I3R3 View 3D Structure Click here
A4V0X6 View 3D Structure Click here
A6NDL7 View 3D Structure Click here
A7IQW5 View 3D Structure Click here
A9JRB6 View 3D Structure Click here
B0K012 View 3D Structure Click here
B3DIQ7 View 3D Structure Click here
B4FEA1 View 3D Structure Click here
B4G0M2 View 3D Structure Click here
B6UDN2 View 3D Structure Click here
B9F4I3 View 3D Structure Click here
C6S3D3 View 3D Structure Click here
C6TP10 View 3D Structure Click here
D3YWP0 View 3D Structure Click here
D3Z8P8 View 3D Structure Click here
D3Z9R2 View 3D Structure Click here
D3ZJG1 View 3D Structure Click here
D4A8Z5 View 3D Structure Click here
F1M0X4 View 3D Structure Click here
F1RBM2 View 3D Structure Click here
F4HQD3 View 3D Structure Click here
F4HUD6 View 3D Structure Click here
F4IVK3 View 3D Structure Click here
F4JNX3 View 3D Structure Click here
I1JCG4 View 3D Structure Click here
I1JCQ3 View 3D Structure Click here
I1LL11 View 3D Structure Click here
I1LR76 View 3D Structure Click here
I1M945 View 3D Structure Click here
I1MCP1 View 3D Structure Click here
I1MN55 View 3D Structure Click here
I1NC14 View 3D Structure Click here
K7K469 View 3D Structure Click here
K7MH00 View 3D Structure Click here
K7MIT7 View 3D Structure Click here
K7MNF0 View 3D Structure Click here
K7U7C5 View 3D Structure Click here
M0R8E0 View 3D Structure Click here
O13926 View 3D Structure Click here
O14118 View 3D Structure Click here
O64850 View 3D Structure Click here
P0C5J1 View 3D Structure Click here
P30643 View 3D Structure Click here
P38347 View 3D Structure Click here
P40389 View 3D Structure Click here
P47163 View 3D Structure Click here
P53970 View 3D Structure Click here
P87241 View 3D Structure Click here
Q05874 View 3D Structure Click here
Q10B95 View 3D Structure Click here
Q10P78 View 3D Structure Click here
Q21069 View 3D Structure Click here
Q23195 View 3D Structure Click here
Q3U2J5 View 3D Structure Click here
Q3UZW7 View 3D Structure Click here
Q4DAX6 View 3D Structure Click here
Q4DFF0 View 3D Structure Click here
Q4DXD2 View 3D Structure Click here
Q4E1L9 View 3D Structure Click here
Q4KM84 View 3D Structure Click here
Q54CB1 View 3D Structure Click here
Q54CB1 View 3D Structure Click here
Q54EJ4 View 3D Structure Click here
Q54F12 View 3D Structure Click here
Q54IN5 View 3D Structure Click here
Q54KW9 View 3D Structure Click here
Q54NK9 View 3D Structure Click here
Q54UI5 View 3D Structure Click here
Q55BF7 View 3D Structure Click here
Q55DL2 View 3D Structure Click here
Q5A013 View 3D Structure Click here
Q5AD01 View 3D Structure Click here
Q5BLD8 View 3D Structure Click here
Q5RJL2 View 3D Structure Click here
Q5VMF1 View 3D Structure Click here
Q5VZV1 View 3D Structure Click here
Q6AUG1 View 3D Structure Click here
Q6DHC5 View 3D Structure Click here
Q6YUS4 View 3D Structure Click here
Q7X8M4 View 3D Structure Click here
Q7XHY7 View 3D Structure Click here
Q7XIH8 View 3D Structure Click here
Q7XQE5 View 3D Structure Click here
Q7Z624 View 3D Structure Click here
Q84TD5 View 3D Structure Click here
Q86JB0 View 3D Structure Click here
Q86JB0 View 3D Structure Click here
Q86XA0 View 3D Structure Click here
Q8BLU2 View 3D Structure Click here
Q8C436 View 3D Structure Click here
Q8CDZ2 View 3D Structure Click here
Q8IHT8 View 3D Structure Click here
Q8N7N1 View 3D Structure Click here
Q8R1C6 View 3D Structure Click here
Q8VY62 View 3D Structure Click here
Q8WXB1 View 3D Structure Click here
Q96AZ1 View 3D Structure Click here
Q96G04 View 3D Structure Click here
Q9BUU2 View 3D Structure Click here
Q9CQL0 View 3D Structure Click here
Q9FFH2 View 3D Structure Click here
Q9FGZ0 View 3D Structure Click here
Q9H867 View 3D Structure Click here
Q9SVL4 View 3D Structure Click here
Q9UT28 View 3D Structure Click here
Q9VEZ4 View 3D Structure Click here
Q9VP86 View 3D Structure Click here
Q9VQK8 View 3D Structure Click here
Q9VWJ9 View 3D Structure Click here
R4GEX7 View 3D Structure Click here
X1WE47 View 3D Structure Click here