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15  structures 1042  species 0  interactions 6263  sequences 518  architectures

Family: Malectin (PF11721)

Summary: Malectin domain

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Malectin domain Provide feedback

Malectin is a membrane-anchored protein of the endoplasmic reticulum that recognises and binds Glc2-N-glycan. It carries a signal peptide from residues 1-26, a C-terminal transmembrane helix from residues 255-274, and a highly conserved central part of approximately 190 residues followed by an acidic, glutamate-rich region. Carbohydrate-binding is mediated by the four aromatic residues, Y67, Y89, Y116, and F117 and the aspartate at D186. NMR-based ligand-screening studies has shown binding of the protein to maltose and related oligosaccharides, on the basis of which the protein has been designated "malectin", and its endogenous ligand is found to be Glc2-high-mannose N-glycan [1].

Literature references

  1. Schallus T, Jaeckh C, Feher K, Palma AS, Liu Y, Simpson JC, Mackeen M, Stier G, Gibson TJ, Feizi T, Pieler T, Muhle-Goll C; , Mol Biol Cell. 2008;19:3404-3414.: Malectin: a novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation. PUBMED:18524852 EPMC:18524852


Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR021720

This entry represents a malectin domain, and can also be found in probable receptor-like serine/threonine-protein kinases from plants [ PUBMED:20064227 ] and in proteins described as glycoside hydrolases.

Malectin is a membrane-anchored protein of the endoplasmic reticulum that recognises and binds Glc2-N-glycan. It carries a signal peptide from residues 1-26, a C-terminal transmembrane helix from residues 255-274, and a highly conserved central part of approximately 190 residues followed by an acidic, glutamate-rich region. Carbohydrate-binding is mediated by the four aromatic residues, Y67, Y89, Y116, and F117 and the aspartate at D186. NMR-based ligand-screening studies has shown binding of the protein to maltose and related oligosaccharides, on the basis of which the protein has been designated "malectin", and its endogenous ligand is found to be Glc2-high-mannose N-glycan [ PUBMED:18524852 ].

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

This family is a member of clan GBD (CL0202), which has the following description:

This large superfamily contains beta sandwich domains with a jelly roll topology. Many of these families are involved in carbohydrate recognition. Despite sharing little sequence similarity they do share a weak sequence motif, with a conserved bulge in the C-terminal beta sheet. The probable role of this bulge is in bending of the beta sheet that contains the bulge. This enables the curvature of the sheet forming the sugar binding site [1].

The clan contains the following 70 members:

7TMR-DISMED2 Agarase_CBM Allantoicase ANAPC10 Arabino_trans_C Bac_rhamnosid_N BcsB BetaGal_dom4_5 BPA_C Calpain_III CBM-like CBM27 CBM32 CBM46 CBM60 CBM65_1 CBM_11 CBM_15 CBM_17_28 CBM_26 CBM_35 CBM_4_9 CBM_6 CE2_N CIA30 Clenterotox Cry1Ac_D5 DUF4465 DUF4627 DUF5000 DUF5010_C DUF5077 DUF5625 DUF642 Endotoxin_C Ephrin_lbd Exop_C F5_F8_type_C FBA FlhE GH101_N GH115_C Glft2_N Glyco_hydro_2_N GxDLY HA70_C Laminin_B Laminin_N Lectin_like Lipl32 Lyase_N Malectin Malectin_like Muskelin_N NPCBM P_proprotein PA-IL PAW PCMD PepX_C PINIT PITH PPC PulA_N1 Sad1_UNC SGBP_B_XBD TcA_TcB_BD Thioredoxin_10 XRCC1_N YpM

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(31)
Full
(6263)
Representative proteomes UniProt
(11855)
RP15
(624)
RP35
(3005)
RP55
(5383)
RP75
(7758)
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PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(31)
Full
(6263)
Representative proteomes UniProt
(11855)
RP15
(624)
RP35
(3005)
RP55
(5383)
RP75
(7758)
Alignment:
Format:
Order:
Sequence:
Gaps:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(31)
Full
(6263)
Representative proteomes UniProt
(11855)
RP15
(624)
RP35
(3005)
RP55
(5383)
RP75
(7758)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Pfam-B_783 (release 23.0) pdb_2jwp
Previous IDs: none
Type: Domain
Sequence Ontology: SO:0000417
Author: Finn RD , Coggill P
Number in seed: 31
Number in full: 6263
Average length of the domain: 166.80 aa
Average identity of full alignment: 31 %
Average coverage of the sequence by the domain: 20.79 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 23.0 23.0
Trusted cut-off 23.0 23.0
Noise cut-off 22.9 22.9
Model length: 165
Family (HMM) version: 10
Download: download the raw HMM for this family

Species distribution

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Archea Archea Eukaryota Eukaryota
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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Malectin domain has been found. There are 15 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A0N7KJP8 View 3D Structure Click here
A0A0N7KKF3 View 3D Structure Click here
A0A0P0W899 View 3D Structure Click here
A0A0P0WET4 View 3D Structure Click here
A0A0P0WET5 View 3D Structure Click here
A0A0P0WJV8 View 3D Structure Click here
A0A0P0XDF2 View 3D Structure Click here
A0A0R0GUE9 View 3D Structure Click here
A0A0R0H9A9 View 3D Structure Click here
A0A0R0HG00 View 3D Structure Click here
A0A0R0IRF6 View 3D Structure Click here
A0A0R0KQX8 View 3D Structure Click here
A0A0R0LGH5 View 3D Structure Click here
A0A1D6DZD5 View 3D Structure Click here
A0A1D6DZD8 View 3D Structure Click here
A0A1D6H6E9 View 3D Structure Click here
A0A1D6I2K1 View 3D Structure Click here
A0A1D6IYJ0 View 3D Structure Click here
A0A1D6JEC3 View 3D Structure Click here
A0A1D6JEH7 View 3D Structure Click here
A0A1D6JL54 View 3D Structure Click here
A0A1D6LLR5 View 3D Structure Click here
A0A1P8AUL5 View 3D Structure Click here
A0A368UHF3 View 3D Structure Click here
A0A368UIM5 View 3D Structure Click here
A9C3P0 View 3D Structure Click here
B9FAF3 View 3D Structure Click here
C0LGE0 View 3D Structure Click here
C0LGG7 View 3D Structure Click here
C0LGG8 View 3D Structure Click here
C0LGG9 View 3D Structure Click here
C0LGH2 View 3D Structure Click here
C0LGH3 View 3D Structure Click here
C0LGN2 View 3D Structure Click here
C6ZRT2 View 3D Structure Click here
F4I337 View 3D Structure Click here
F4I3K0 View 3D Structure Click here
F4I3K4 View 3D Structure Click here
F4IBQ9 View 3D Structure Click here
F4IJK6 View 3D Structure Click here
I1J4Y9 View 3D Structure Click here
I1JJ63 View 3D Structure Click here
I1K437 View 3D Structure Click here
I1KW58 View 3D Structure Click here
I1L2F0 View 3D Structure Click here
I1LV96 View 3D Structure Click here
I1M1N4 View 3D Structure Click here
I1M2X3 View 3D Structure Click here
I1M6V6 View 3D Structure Click here
K7K3B8 View 3D Structure Click here
K7KWQ9 View 3D Structure Click here
K7KX43 View 3D Structure Click here
K7LEZ9 View 3D Structure Click here
K7LUE0 View 3D Structure Click here
K7LV51 View 3D Structure Click here
K7M246 View 3D Structure Click here
K7M247 View 3D Structure Click here
Q14165 View 3D Structure Click here
Q5FVQ4 View 3D Structure Click here
Q5VRG4 View 3D Structure Click here
Q6EQG8 View 3D Structure Click here
Q6EUF7 View 3D Structure Click here
Q6ZKW6 View 3D Structure Click here
Q6ZKW7 View 3D Structure Click here
Q6ZQI3 View 3D Structure Click here
Q7XTP6 View 3D Structure Click here
Q95QG1 View 3D Structure Click here
Q9ASQ6 View 3D Structure Click here
Q9FXF2 View 3D Structure Click here
Q9VIG0 View 3D Structure Click here