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161  structures 3338  species 0  interactions 33086  sequences 1606  architectures

Family: EF-hand_5 (PF13202)

Summary: EF hand

Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.

This is the Wikipedia entry entitled "EF hand". More...

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.

This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.

EF hand Provide feedback

No Pfam abstract.

Internal database links

This tab holds annotation information from the InterPro database.

InterPro entry IPR002048

Many calcium-binding proteins belong to the same evolutionary family and share a type of calcium-binding domain known as the EF-hand. This type of domain consists of a twelve residue loop flanked on both sides by a twelve residue alpha-helical domain. In an EF-hand loop the calcium ion is coordinated in a pentagonal bipyramidal configuration. The six residues involved in the binding are in positions 1, 3, 5, 7, 9 and 12; these residues are denoted by X, Y, Z, -Y, -X and -Z. The invariant Glu or Asp at position 12 provides two oxygens for liganding Ca (bidentate ligand). Ca2 binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers [ PUBMED:9228939 , PUBMED:8848832 , PUBMED:7553064 , PUBMED:7656053 , PUBMED:10591109 ].

Gene Ontology

The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.

Domain organisation

Below is a listing of the unique domain organisations or architectures in which this domain is found. More...

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Pfam Clan

Alignments

We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database (reference proteomes) using the family HMM. We also generate alignments using four representative proteomes (RP) sets and the UniProtKB sequence database. More...

View options

We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.

  Seed
(89)
Full
(33086)
Representative proteomes UniProt
(103959)
RP15
(6949)
RP35
(16292)
RP55
(30048)
RP75
(42123)
Jalview View  View  View  View  View  View  View 
HTML View             
PP/heatmap 1            

1Cannot generate PP/Heatmap alignments for seeds; no PP data available

Key: ✓ available, x not generated, not available.

Format an alignment

  Seed
(89)
Full
(33086)
Representative proteomes UniProt
(103959)
RP15
(6949)
RP35
(16292)
RP55
(30048)
RP75
(42123)
Alignment:
Format:
Order:
Sequence:
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Download options

We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.

  Seed
(89)
Full
(33086)
Representative proteomes UniProt
(103959)
RP15
(6949)
RP35
(16292)
RP55
(30048)
RP75
(42123)
Raw Stockholm Download   Download   Download   Download   Download   Download   Download  
Gzipped Download   Download   Download   Download   Download   Download   Download  

You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.

HMM logo

HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...

Trees

This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.

Note: You can also download the data file for the tree.

Curation and family details

This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.

Curation View help on the curation process

Seed source: Jackhmmer:O26668
Previous IDs: EF_hand_3;
Type: Domain
Sequence Ontology: SO:0000417
Author: Bateman A
Number in seed: 89
Number in full: 33086
Average length of the domain: 23.20 aa
Average identity of full alignment: 30 %
Average coverage of the sequence by the domain: 8.02 %

HMM information View help on HMM parameters

HMM build commands:
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 25.9 11.5
Trusted cut-off 25.9 11.5
Noise cut-off 25.8 11.4
Model length: 25
Family (HMM) version: 8
Download: download the raw HMM for this family

Species distribution

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Selections

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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

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Structures

For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the EF-hand_5 domain has been found. There are 161 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.

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AlphaFold Structure Predictions

The list of proteins below match this family and have AlphaFold predicted structures. Click on the protein accession to view the predicted structure.

Protein Predicted structure External Information
A0A096QYB9 View 3D Structure Click here
A0A0G2K2W5 View 3D Structure Click here
A0A0G2K952 View 3D Structure Click here
A0A0G2K952 View 3D Structure Click here
A0A0G2KAU4 View 3D Structure Click here
A0A0G2KAU4 View 3D Structure Click here
A0A0G2KVE1 View 3D Structure Click here
A0A0G2KVE1 View 3D Structure Click here
A0A0H2UKS1 View 3D Structure Click here
A0A0H2UKS1 View 3D Structure Click here
A0A0P0VG45 View 3D Structure Click here
A0A0P0VGM2 View 3D Structure Click here
A0A0P0VGM2 View 3D Structure Click here
A0A0P0VYA3 View 3D Structure Click here
A0A0P0X1C3 View 3D Structure Click here
A0A0P0Y9X3 View 3D Structure Click here
A0A0R0FB11 View 3D Structure Click here
A0A0R0FHW7 View 3D Structure Click here
A0A0R0FHW7 View 3D Structure Click here
A0A0R0HP89 View 3D Structure Click here
A0A0R0JW45 View 3D Structure Click here
A0A0R0KFN4 View 3D Structure Click here
A0A178V266 View 3D Structure Click here
A0A178V266 View 3D Structure Click here
A0A1D6E6R4 View 3D Structure Click here
A0A1D6EFQ0 View 3D Structure Click here
A0A1D6F1T5 View 3D Structure Click here
A0A1D6FUZ5 View 3D Structure Click here
A0A1D6GWT6 View 3D Structure Click here
A0A1D6HD01 View 3D Structure Click here
A0A1D6J2E1 View 3D Structure Click here
A0A1D6J9N5 View 3D Structure Click here
A0A1D6JI85 View 3D Structure Click here
A0A1D6JMN9 View 3D Structure Click here
A0A1D6KIG5 View 3D Structure Click here
A0A1D6KIG5 View 3D Structure Click here
A0A1D6LUR9 View 3D Structure Click here
A0A1D6MA49 View 3D Structure Click here
A0A1D6MA49 View 3D Structure Click here
A0A1D6MVT0 View 3D Structure Click here
A0A1D6MVT1 View 3D Structure Click here
A0A1D6NKC2 View 3D Structure Click here
A0A1D6P328 View 3D Structure Click here
A0A1D6PJG6 View 3D Structure Click here
A0A1D6QMM1 View 3D Structure Click here
A0A1D6QNN9 View 3D Structure Click here
A0A1D6QNN9 View 3D Structure Click here
A0A1P8AYB6 View 3D Structure Click here
A0A1X7YH95 View 3D Structure Click here
A0A2R8PZX9 View 3D Structure Click here
A0A2R8Q6S0 View 3D Structure Click here
A0A2R8Q977 View 3D Structure Click here
A0A2R8QA27 View 3D Structure Click here
A0A2R8QCR4 View 3D Structure Click here
A0A2R8QMH0 View 3D Structure Click here
A0A2R8QMH0 View 3D Structure Click here
A0A494BAW1 View 3D Structure Click here
A2VEI2 View 3D Structure Click here
A4HVS3 View 3D Structure Click here
A4HVS3 View 3D Structure Click here
A4I620 View 3D Structure Click here
A4I620 View 3D Structure Click here
A4IA71 View 3D Structure Click here
A4IG32 View 3D Structure Click here
B1AUS6 View 3D Structure Click here
B1WC34 View 3D Structure Click here
B4FRR5 View 3D Structure Click here
B4FRR5 View 3D Structure Click here
B4G1S0 View 3D Structure Click here
B6SL16 View 3D Structure Click here
B6TEH6 View 3D Structure Click here
B6TXZ0 View 3D Structure Click here
B6TYU9 View 3D Structure Click here
C0H4U9 View 3D Structure Click here
C0H4U9 View 3D Structure Click here
C4J3M8 View 3D Structure Click here
C6T243 View 3D Structure Click here
D3ZID7 View 3D Structure Click here
D3ZTW5 View 3D Structure Click here
D3ZUB0 View 3D Structure Click here
D3ZUB0 View 3D Structure Click here
D3ZUB0 View 3D Structure Click here
D3ZZK0 View 3D Structure Click here
D6W196 View 3D Structure Click here
E7F646 View 3D Structure Click here
E7F7H4 View 3D Structure Click here
E7FCR5 View 3D Structure Click here
F1LZH7 View 3D Structure Click here
F1Q5Y8 View 3D Structure Click here
F1Q7B2 View 3D Structure Click here
F1QUS0 View 3D Structure Click here
F1R369 View 3D Structure Click here
F1R369 View 3D Structure Click here
F1R4I7 View 3D Structure Click here
F1R763 View 3D Structure Click here
F4HY32 View 3D Structure Click here
F8VPZ3 View 3D Structure Click here
F8VPZ3 View 3D Structure Click here
G3V7W1 View 3D Structure Click here
G5EBH7 View 3D Structure Click here
G5EBH7 View 3D Structure Click here
G5EBX9 View 3D Structure Click here
G5EBX9 View 3D Structure Click here
G5EC87 View 3D Structure Click here
H2KY97 View 3D Structure Click here
H2KY97 View 3D Structure Click here
I1JD04 View 3D Structure Click here
I1JN81 View 3D Structure Click here
I1JST7 View 3D Structure Click here
I1K7B7 View 3D Structure Click here
I1K7Z2 View 3D Structure Click here
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I1KGD3 View 3D Structure Click here
I1KMQ0 View 3D Structure Click here
I1KPK9 View 3D Structure Click here
I1KUV0 View 3D Structure Click here
I1KY83 View 3D Structure Click here
I1LA46 View 3D Structure Click here
I1LFA4 View 3D Structure Click here
I1LLG5 View 3D Structure Click here
I1LPH9 View 3D Structure Click here
I1LPH9 View 3D Structure Click here
I1LXD2 View 3D Structure Click here
I1M4C2 View 3D Structure Click here
I1M635 View 3D Structure Click here
I1MIA8 View 3D Structure Click here
I1MIA8 View 3D Structure Click here
I1MIH4 View 3D Structure Click here
I1MUN9 View 3D Structure Click here
I1MUN9 View 3D Structure Click here
I1MVY3 View 3D Structure Click here
I1N833 View 3D Structure Click here
I1NEN3 View 3D Structure Click here
I1NF42 View 3D Structure Click here
I6L9G5 View 3D Structure Click here
I6L9G5 View 3D Structure Click here
K7KHY2 View 3D Structure Click here
K7KIG8 View 3D Structure Click here
K7KRW8 View 3D Structure Click here
K7KRW8 View 3D Structure Click here
K7KRW8 View 3D Structure Click here
K7KSY7 View 3D Structure Click here
K7KSY7 View 3D Structure Click here
K7LJ63 View 3D Structure Click here
K7LRI6 View 3D Structure Click here
K7M288 View 3D Structure Click here
K7M5L0 View 3D Structure Click here
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K7MF78 View 3D Structure Click here
K7MN50 View 3D Structure Click here
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K7UIL7 View 3D Structure Click here
K7UU06 View 3D Structure Click here
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K7V294 View 3D Structure Click here
K7VJ62 View 3D Structure Click here
K7VJ62 View 3D Structure Click here
K7W555 View 3D Structure Click here
M0RCD9 View 3D Structure Click here
M9PD06 View 3D Structure Click here
M9PD06 View 3D Structure Click here
O08795 View 3D Structure Click here
O17068 View 3D Structure Click here
O35783 View 3D Structure Click here
O35887 View 3D Structure Click here
O35887 View 3D Structure Click here
O43745 View 3D Structure Click here
O75340 View 3D Structure Click here
O76038 View 3D Structure Click here
O76670 View 3D Structure Click here
O81445 View 3D Structure Click here
P14314 View 3D Structure Click here
P41044 View 3D Structure Click here
P51879 View 3D Structure Click here
P53684 View 3D Structure Click here
Q02045 View 3D Structure Click here
Q05186 View 3D Structure Click here
Q05186 View 3D Structure Click here
Q05186 View 3D Structure Click here
Q0E8F2 View 3D Structure Click here
Q0J0U3 View 3D Structure Click here
Q0J0U3 View 3D Structure Click here
Q0J8Y3 View 3D Structure Click here
Q10S92 View 3D Structure Click here
Q13938 View 3D Structure Click here
Q14257 View 3D Structure Click here
Q14257 View 3D Structure Click here
Q14257 View 3D Structure Click here
Q15293 View 3D Structure Click here
Q15293 View 3D Structure Click here
Q18887 View 3D Structure Click here
Q1ZXH5 View 3D Structure Click here
Q2QY10 View 3D Structure Click here
Q2RAR8 View 3D Structure Click here
Q3HRN8 View 3D Structure Click here
Q3HRN9 View 3D Structure Click here
Q3HRP2 View 3D Structure Click here
Q42438 View 3D Structure Click here
Q4CVY2 View 3D Structure Click here
Q4D2E0 View 3D Structure Click here
Q4D3P2 View 3D Structure Click here
Q4D6V6 View 3D Structure Click here
Q4D6X9 View 3D Structure Click here
Q4D6X9 View 3D Structure Click here
Q4DGP9 View 3D Structure Click here
Q4DQ24 View 3D Structure Click here
Q4V8A1 View 3D Structure Click here
Q502J0 View 3D Structure Click here
Q54JS1 View 3D Structure Click here
Q54JS1 View 3D Structure Click here
Q54NH0 View 3D Structure Click here
Q54RF4 View 3D Structure Click here
Q54RF4 View 3D Structure Click here
Q5PNM8 View 3D Structure Click here
Q5U2V1 View 3D Structure Click here
Q5U2V1 View 3D Structure Click here
Q5XJX1 View 3D Structure Click here
Q61112 View 3D Structure Click here
Q61576 View 3D Structure Click here
Q61576 View 3D Structure Click here
Q62703 View 3D Structure Click here
Q62703 View 3D Structure Click here
Q62703 View 3D Structure Click here
Q641Z8 View 3D Structure Click here
Q64691 View 3D Structure Click here
Q66H94 View 3D Structure Click here
Q67J27 View 3D Structure Click here
Q6DG81 View 3D Structure Click here
Q6IQP3 View 3D Structure Click here
Q6IQP3 View 3D Structure Click here
Q6NZH9 View 3D Structure Click here
Q6NZH9 View 3D Structure Click here
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Q6R556 View 3D Structure Click here
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Q7LDG7 View 3D Structure Click here
Q7SXV9 View 3D Structure Click here
Q7SXV9 View 3D Structure Click here
Q7YTK4 View 3D Structure Click here
Q7YTK4 View 3D Structure Click here
Q7Z2B8 View 3D Structure Click here
Q801M3 View 3D Structure Click here
Q804W1 View 3D Structure Click here
Q869S9 View 3D Structure Click here
Q86A72 View 3D Structure Click here
Q86IV8 View 3D Structure Click here
Q86JE1 View 3D Structure Click here
Q86JE1 View 3D Structure Click here
Q8BH97 View 3D Structure Click here
Q8BH97 View 3D Structure Click here
Q8BP92 View 3D Structure Click here
Q8IID5 View 3D Structure Click here
Q8IID5 View 3D Structure Click here
Q8IIR7 View 3D Structure Click here
Q8IIR7 View 3D Structure Click here
Q8ILR3 View 3D Structure Click here
Q8ILR3 View 3D Structure Click here
Q8LAS7 View 3D Structure Click here
Q8NFA0 View 3D Structure Click here
Q8NFA0 View 3D Structure Click here
Q8VCX5 View 3D Structure Click here
Q8W4L0 View 3D Structure Click here
Q91WD9 View 3D Structure Click here
Q91ZS3 View 3D Structure Click here
Q95PZ2 View 3D Structure Click here
Q95Y96 View 3D Structure Click here
Q95YL4 View 3D Structure Click here
Q95YL5 View 3D Structure Click here
Q966Q9 View 3D Structure Click here
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Q96AY3 View 3D Structure Click here
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Q96P71 View 3D Structure Click here
Q96PH1 View 3D Structure Click here
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Q9LM78 View 3D Structure Click here
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Q9LY28 View 3D Structure Click here
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Q9UU93 View 3D Structure Click here
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Q9Y680 View 3D Structure Click here
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Q9Z247 View 3D Structure Click here
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