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8  structures 233  species 0  interactions 299  sequences 16  architectures

Family: Plk4_PB1 (PF18190)

Summary: Polo-like Kinase 4 Polo Box 1

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This is the Wikipedia entry entitled "Polo-like kinase". More...

Polo-like kinase Edit Wikipedia article

Polo-like kinases (Plks) are regulatory serine/threonin kinases of the cell cycle involved in mitotic entry, mitotic exit, spindle formation, cytokinesis, and meiosis.[1] Only one Plk is found in the genomes of fruit flies (Polo), budding yeast (Cdc5) and fission yeast (Plo1).[1] Vertebrates, however, have many Plk family members including Plk1 (Xenopus Plx1), Plk2/Snk (Xenopus Plx2), Plk3/Prk/FnK (Xenopus Plx3), Plk4/Sak and Plk5.[1] Of the vertebrate Plk family members, the mammalian Plk1 has been most extensively studied. During mitosis and cytokinesis, Plks associate with several structures including the centrosome, kinetochores, and the central spindle.

Structure

The catalytic serine/threonine kinase domain of Plk is at the N-terminus of the polo-like kinase protein.[1] A regulatory domain containing two signature motifs, known as polo box domains, is located at the C-terminus.[1] The polo-box domain (PBD) helps with specificity of substrate and localizes Plk to specific mitotic structures during mitosis.[1] These include the centrosomes in early M phase, the spindle midzone in early and late anaphase and the midbody during cytokinesis.[2]

Regulation

Plks are controlled at the level of protein synthesis and degradation, by the action of upstream kinases and phosphatases, and by localization to specific subcellular structures. Plks are activated by phosphorylation within a short region of the catalytic domain called the T-loop (or activation-loop), with several serine/threonine phosphorylation sites in the loop identified.[3] Polo-like kinase kinase 1 (Plkk1) and protein kinase A (PKA) have been shown to be able to phosphorylate Plk1 in vitro[4]. The polo-box domain (PBD) of Plk1 is a phosphopeptide-binding motif.[5] This means that in the absence of a phosphorylated ligand, the PBD interacts with the catalytic domain thereby preventing substrate binding or kinase activation. Occupancy of the PBD by an exogenous phosphopeptide ligand would then cause the release of the catalytic domain, which, together with phosphorylation on the T-loop, converts Plk to the active form. On exit from mitosis, Plks are proteolytically degraded through the ubiquitin-proteasome pathway after coming in contact with the ubiquitin-ligase Anaphase Promoting Complex (APC).[6]

Mitosis

Plks have been found to cooperate with Cdks in the orchestration of cell division. Entry into M phase is controlled through the activation of cyclin-dependent kinase 1 (CDK1)–cyclin B, and Cdc25 is a phosphatase that dephosphorylates Cdk1 to promote mitotic entry. Plk1 binds to phosphorylated Cdc25 through its PBD.[7] Thus Plks can phosphorylate Cdc25 and thereby regulate Cdc25 and indirectly Cdk1. A study shows that phosphorylation of a serine residue (Ser198) within a nuclear-export signal of Cdc25 promotes the nuclear accumulation of Ccdc25 in humans.[8] PBD has high affinity for proteins already phosphorylated at certain serine/threonine sites.[3] This requires priming of substrates by Plk itself or other kinases such as Cdk1 to create a docking site. However, there could also be phosphorylation-independent structural aspects contributing to binding. Plo1 (the Plk found in fission yeast) is part of a positive-feedback loop that controls the expression of genes that are required for cell division.

Plk has also been shown to be needed at the G2/M transition. Spindle pole formation needs Plk1, and some proteins such as gamma-tubulin fail to recruit spindle poles in the absence of Plk1 for centrosome maturation. Several other potential Plk1 substrates and binding partners that are implicated in microtubule nucleation and dynamics have also been identified including the microtubule-severing protein katanin,[9] the microtubule-stabilizing protein TCTP[10] and the microtubule-destabilizing protein stathmin.[11]

Plk is also needed for successful chromosome separation and exit from mitosis. Plk cooperates with Cdk1 in the control of several APC subunits.[3] Human PLK1 phosphorylates early mitotic inhibitor 1 (EMI1), an inhibitor of the APC.[12] Impairment of Plk function generally interferes with the normal onset of anaphase, indicating that Plks contribute to the control of APC activity. Plk1 associates with kinetochores during mitosis. In the absence of Plk1 function, bipolar spindle formation does not occur and cells arrest in prometaphase owing to Spindle Assembly Checkpoint activation. Plk1 function may be important for relieving the inhibitory checkpoint signal. If so, Plk1 could contribute to the resumption of mitotic progression on complete attachment of all chromosomes to the spindle apparatus.

Meiosis

The fly and yeast models have revealed that Polo kinases coordinate the more complex pattern of chromosome segregation in meiosis. Budding yeast Cdc5 is required in meiosis I for the removal of cohesins from chromosome arms, for the co-orientation of homologous chromosomes, and for the resolution of crossovers.[13] Cdc5 (Plk found in budding yeast) directly phosphorylates the meiotic cohesin and promotes its dissociation from the chromosome arms to allow for recombination but not from the centromeric region in meoisis I. In some yeast cdc5 mutants, bipolar rather than monopolar attachment of the sister kinetochores occurs during meiosis I because a complex of proteins called monopolins fails to localize to the kinetochore.[14]

Cytokinesis

The involvement of Polo kinases in the process of cytokinesis was first shown in fission yeast, in which the overexpression of Plo1 drives septation at any stage of the cell cycle and plo1 mutants fail to septate.[15] A protein called Mid1 determines where the contractile ring forms has been shown to shuttle out of the nucleus due to phosphorylation of Plk.[16] Recent studies on the role of mammalian Plk1 in cytokinesis have also identified kinesin-related motor Mklp2 and dynein subcomponent NudC as potential substrates of Plk1 that interact with the PBD.[17] Both Mklp2 and NudC have associated motor-protein activity and both localize to the central spindle. PLK1 has been found to phosphorylate the centralspindlin subunit CYK4 at the spindle midzone, thereby allowing the recruitment of the Rho guanine nucleotide-exchange factor (GEF) ECT2 to promote RhoA activation and thus actomyosin contraction of the ring.[18]

See also

References

  1. ^ a b c d e f Barr, Francis A., Herman HW Silljé, and Erich A. Nigg. "Polo-like kinases and the orchestration of cell division." Nature reviews Molecular cell biology5.6 (2004): 429-441.
  2. ^ Fenton, Brian; Glover, David M. (1993). "A conserved mitotic kinase active at late anaphase—telophase in syncytial Drosophila embryos". Nature. 363 (6430): 637–40. Bibcode:1993Natur.363..637F. doi:10.1038/363637a0. PMID 8510757. 
  3. ^ a b c Archambault, Vincent, and David M. Glover. "Polo-like kinases: conservation and divergence in their functions and regulation." Nature reviews Molecular cell biology 10.4 (2009): 265-275.
  4. ^ Qian, Y. W., Erikson, E. & Maller, J. L. Purification and cloning of a protein kinase that phosphorylates and activates the polo-like kinase Plx1. Science 282, 1701–1704 (1998).
  5. ^ Elia, A. E., Cantley, L. C. & Yaffe, M. B. Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates. Science 299, 1228–1231 (2003).
  6. ^ Shirayama, M., Zachariae, W., Ciosk, R. & Nasmyth, K. The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J. 17, 1336–1349 (1998).
  7. ^ Kumagai, A. & Dunphy, W. G. Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts. Science 273, 1377–1380 (1996).
  8. ^ Toyoshima-Morimoto, F., Taniguchi, E. & Nishida, E. Plk1 promotes nuclear translocation of human Cdc25C during prophase. EMBO Rep. 3, 341–348 (2002).
  9. ^ McNally, K. P., Buster, D. & McNally, F. J. Katanin-mediated microtubule severing can be regulated by multiple mechanisms. Cell Motil. Cytoskeleton 53, 337–349 (2002).
  10. ^ Yarm, F. R. Plk phosphorylation regulates the microtubulestabilizing protein TCTP. Mol. Cell. Biol. 22, 6209–6221 (2002).
  11. ^ Budde, P. P., Kumagai, A., Dunphy, W. G. & Heald, R. Regulation of Op18 during spindle assembly in Xenopus egg extracts. J. Cell Biol. 153, 149–158 (2001).
  12. ^ Hansen, D. V., Loktev, A. V., Ban, K. H. & Jackson, P. K. Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFTrCP-dependent destruction of the APC inhibitor Emi1. Mol. Biol. Cell 15, 5623–5634 (2004).
  13. ^ Alexandru, G., Uhlmann, F., Mechtler, K., Poupart, M. A. & Nasmyth, K. Phosphorylation of the cohesin subunit Scc1 by Polo/Cdc5 kinase regulates sister chromatid separation in yeast. Cell 105, 459–472 (2001).
  14. ^ Clyne, R. K. et al. Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I. Nature Cell Biol. 5, 480–485 (2003).
  15. ^ Mulvihill, D. P., Petersen, J., Ohkura, H., Glover, D. M. & Hagan, I. M. Plo1 kinase recruitment to the spindle pole body and its role in cell division in Schizosaccharomyces pombe. Mol. Biol. Cell 10, 2771–2785 (1999).
  16. ^ Bahler, J. et al. Role of polo kinase and Mid1p in determining the site of cell division in fission yeast. J. Cell Biol. 143, 1603–1616 (1998).
  17. ^ Neef, R. et al. Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis. J. Cell Biol. 162, 863–875 (2003).
  18. ^ Yoshida, S. et al. Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis. Science 313, 108–111 (2006).

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Polo-like Kinase 4 Polo Box 1 Provide feedback

This domain is found in Polo-like kinase 4 (Plk4) present in Drosophila melanogaster. Plk4 is a conserved component in the duplication pathway of centrioles which is needed to prevent chromosomal instability. The domain is Polo Box 1 (PB1) and has a pseudo-symmetric dimerization interface across PB1-PB1 [1].

Literature references

  1. Slevin LK, Nye J, Pinkerton DC, Buster DW, Rogers GC, Slep KC;, Structure. 2012;20:1905-1917.: The structure of the plk4 cryptic polo box reveals two tandem polo boxes required for centriole duplication. PUBMED:23000383 EPMC:23000383


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  Seed
(11)
Full
(299)
Representative proteomes UniProt
(460)
NCBI
(809)
Meta
(0)
RP15
(86)
RP35
(160)
RP55
(232)
RP75
(266)
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This family is new in this Pfam release.

Seed source: ECOD:EUF01979
Previous IDs: Plk1_PB1;
Type: Domain
Sequence Ontology: SO:0000417
Author: Smart A
Number in seed: 11
Number in full: 299
Average length of the domain: 103.80 aa
Average identity of full alignment: 42 %
Average coverage of the sequence by the domain: 12.59 %

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build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 45638612 -E 1000 --cpu 4 HMM pfamseq
Model details:
Parameter Sequence Domain
Gathering cut-off 26.4 26.4
Trusted cut-off 26.7 26.4
Noise cut-off 25.9 26.3
Model length: 108
Family (HMM) version: 1
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